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Four E's USA (A Four E's Scientific Company)

Real-Time PCR - PCR200484/PCR200482

● Comes with 48-well design and two models.
● 4-channel 48E can detect up to 4 target types per tube at the same time, while 2-channel 48R can detect up to 2 targets.
● Efficient temperature control with only taking40 minutes to complete a standard PCR amplification program.
● Temperature accuracy is controlled within 0.1℃.
● In 2 seconds, all 48-hole 4(48E)-/2(48R)-color fluorescence scanning can be completed.
● Designed with a built-in 7-inch full-color touch screen, independent control system and USB port for data export with the storage of at least 1000 experiments.
● For the stand-alone operation the device can also be connected to an optional thermal printer for direct printing of sample amplification curves and CT values.
● Compatible with the fluorescence analysis functions of a variety of isothermal amplification technologies.
● Independent data management software built in Gentier 48E/48R for the centralized management and analysis of your massive experimental data, to minimize the errors caused by manual analysis of original data.







Fluorescence Channels



Compatible Fluorophores

Channel 1: FAM, SYBR Green I, etc. Channel 2: VIC, HEX, TET, JOE, etc. Channel 3: ROX, Texas Red, etc.

Channel 4: Cy5, etc.

Channel 1: FAM, SYBR Green I, etc. Channel 2: VIC, HEX, TET, JOE, etc.

Light Source

High-brightness, long-life and maintenance-free LED light source


Photodiodes (PDs)

Fluorescence   Scanning Time

48-well 4-channel detection completed within 2s

48-well 2-channel detection completed   within 2s

Heating Rate

Maximum heating ramp rate ≥8.0℃/s; Average heating ramp rate ≤6.2℃/s

Cooling Rate

Maximum cooling ramp rate ≥6.2℃/s; Average heating ramp rate≥4.5℃/s

Temperature Accuracy

≤ 0.1℃

Special Temperature Setting Function

Support thermal gradients PCR, long PCR, Touch Down PCR

Suitable Consumables

0.2ml clear non-skirt 48-well plates, 8-tube strips, single tubes, etc.

Sample Testing linearity and


linear Correlation: CV≥0.999

Repeatability: cycle threshold (Ct) value CV ≤0.5%

Software Analysis

Qualitative analysis, absolute quantitative analysis, relative quantitative analysis, end point fluorescence analysis, melting curve analysis, and genotyping analysis, etc.

Control Method

Stand-alone operation: 7-inch touchscreen control; Cloud-enabled: PC software control via direct connection or LAN (local area network); Up to 10 devices controlled by one


Power Failure Protection

Automatic recovery of the experiment and other functions when the power is on again after cutting off, without waiting for the power-on of the computer or software control

Data Storage and Transmission

A single machine can store more than 1,000 experimental data files, which can be   imported and exported via USB disks

Stand-Alone Analysis Results

No need for an extra computer: experimental data analysis enabled within the instrument; Direct printing function (optional) to output the sample amplification curve and CT value, simply by connecting to the compatible thermal printer

Reporting Function

Built-in experiment report templates for a variety of industries; Fully open universal reporting of which the contents and formats can be customized

Operating System

Windows XP Pro or higher

Power Supply and Power 


AC 100-240V, 50-60Hz; 600VA


11Kg (net)

Instrument Dimension


Does Four E‘s USA offer Free Shipping in the USA?

We offer FREE GROUND SHIPPING on orders within the contiguous 48 States in the USA (excluding Alaska and Hawaii). No promo code is needed for free shipping! We cannot ship to PO Boxes, so please provide a full, physical street address in your Shipping Address.

What are the Delivery Time Frames?

Unless otherwise noted, orders are processed every business day and are normally delivered within 3-8 business days. Large volumes of items or heavy items may require delivery by freight truck. If your purchase requires this shipping method, we will contact you in advance by email with additional information about freight truck deliveries.

I ordered more than one item. Will they all be delivered at the same time?

We try to make sure your Items ordered together reach you at the same time. Sometimes your items are not always shipped on the same day or in the same carton since different shipping options are used, depending on the product. Once an item has been shipped, you will receive a shipment notification email.

Can I change the delivery address of my package after it has been shipped out?

Unfortunately, we cannot change the shipment once the product is on the way to the delivery address of your order. For more information, please get in touch with our Support Team at at any time.
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About Four E’s

Serving Laboratory Market Worldwide

We design and manufacture laboratory and medical equipment that delivers accurate and reliable performance. Four E's consumables and devices are trusted worldwide in research and medical laboratories. Due to our OEM and Private Label capabilities, "You may not recognize our name, but you've seen our products